Bacterial Fabricated Ultrasonic Sterilization of Culture Media

The study aimed to determine the effect of the fabricated ultrasonic device in sterilizing culture media and inactivating Escherichia coli. The study was conducted using a randomized experimental design. Thioglycollate broth applied with sonic frequencies at 50, 80, and 126 kHz for 15 minutes shows the variability of absorbance readings. The findings revealed that after 15 minutes of treatment, Thioglycolate broth exposed at 80 and 126 kHz is viable to be used as a culture medium to inoculate the organism for microbial analysis, as evidenced by the comparable results of the autoclaved sample. The medium was treated at 50 kHz, and those untreated samples had higher absorbance readings than the autoclave. Sonolysis at 15 minutes of exposure impacts the inactivation of Escherichia coli at 50 kHz, 80, and 126 kHz, as seen in the decrease of the colony count at increasing sonic frequencies. The results revealed a higher mean on unsterilized plates with 126 kHz treatment, with a lesser colony count. Colony count reductions of 6%, 14%, and 29% were obtained at ultrasonic frequencies of 50 kHz, 80 kHz, and 126 kHz, respectively. The majority of the inactivation occurred at 126 kHz exposure.

Keywords: Sonolysis, Thioglycolate, turbidity, kHZ, absorbance, CFU/ml